Fig. 2.
Fig. 2. Surface phenotype of mouse blood pre-DCs. / Mouse blood pre-DCs were enriched as described in “Materials and methods” and in Figure 1, including presorting to eliminate autofluorescent cells. The enriched preparation was then stained for surface expression of CD11c and CD45RA, together with MHC class 2 and one of the additional markers shown. Resultant fluorescence distribution data were gated on either the CD11cloCD45RAhi or the CD11cintCD45RA− pre-DC subgroups, as shown in the top dot plot. Distributions of the other markers are shown in the bottom panels. The broken line gives the background fluorescence with only the relevant stain omitted. Both pre-DC populations were negative for expression of CD4, CD8α, CD80, CD86, and IL-3R (data not shown). Results are representative of 2 to 5 such analyses.

Surface phenotype of mouse blood pre-DCs.

Mouse blood pre-DCs were enriched as described in “Materials and methods” and in Figure 1, including presorting to eliminate autofluorescent cells. The enriched preparation was then stained for surface expression of CD11c and CD45RA, together with MHC class 2 and one of the additional markers shown. Resultant fluorescence distribution data were gated on either the CD11cloCD45RAhi or the CD11cintCD45RA− pre-DC subgroups, as shown in the top dot plot. Distributions of the other markers are shown in the bottom panels. The broken line gives the background fluorescence with only the relevant stain omitted. Both pre-DC populations were negative for expression of CD4, CD8α, CD80, CD86, and IL-3R (data not shown). Results are representative of 2 to 5 such analyses.

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