Fig. 1.
WT1 amplification plot and standard curve.
(A) Amplification plot of a 10-fold serial dilution of the standard K562 cDNA (ranging from 10−5 to 100) and (B) standard curve of K562 cDNA dilution for real-time RT-PCR. The amplification plot shows the increase of reporter fluorescence (ΔRn) during amplification. The Ct value decrease was proportional to the increase of the target molecules. The standard curve shows the linear correlation between the Ct value and the logarithm of the initial concentration of the standard K562 cDNA and can then be used to calculate the relative WT1 gene expression levels in unknown patient samples.
