Fig. 1.
Fig. 1. Kinetics of apoptotic cell uptake by splenic DCs in vivo. / (A) A quantity of 2 × 107 PKH67-labeled BALB/c apoptotic splenocytes was injected intravenously into B10 mice. Internalization of apoptotic cells by DCs was analyzed by flow cytometry at different time points. (B) Splenic DC-enriched suspensions were labeled with phycoerythrin anti-CD11c and Cychrome anti-CD8α mAbs and analyzed by flow cytometry. (C) Expression of the IAb-IEα52-68 peptide detected with Y-Ae mAb on CD11c+ spleen (B10) DCs 24 hours (thick line) or 48 hours (thin line) after intravenous injection of (BALB/c) apoptotic splenocytes. As control, splenic DCs from animals injected with IEα− (B10) apoptotic leukocytes were included (gray histogram). Numbers on dot plots represent percentages of DCs that have internalized PKH67+ apoptotic cells. Data are representative of 4 independent experiments.

Kinetics of apoptotic cell uptake by splenic DCs in vivo.

(A) A quantity of 2 × 107 PKH67-labeled BALB/c apoptotic splenocytes was injected intravenously into B10 mice. Internalization of apoptotic cells by DCs was analyzed by flow cytometry at different time points. (B) Splenic DC-enriched suspensions were labeled with phycoerythrin anti-CD11c and Cychrome anti-CD8α mAbs and analyzed by flow cytometry. (C) Expression of the IAb-IEα52-68 peptide detected with Y-Ae mAb on CD11c+ spleen (B10) DCs 24 hours (thick line) or 48 hours (thin line) after intravenous injection of (BALB/c) apoptotic splenocytes. As control, splenic DCs from animals injected with IEα− (B10) apoptotic leukocytes were included (gray histogram). Numbers on dot plots represent percentages of DCs that have internalized PKH67+ apoptotic cells. Data are representative of 4 independent experiments.

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