Relationship between 8D4⁺ IL-4 production and apoptosis.

(A) Dual anti–IL-4 (MP4 and 8D4 mAb) and 7-AAD staining on CD3⁺ T cells from an HIV-infected patient, after either 24-hour culture in medium to induce deprivation apoptosis, or after induction of primary necrosis by 15-minute hyperthermia at 56°C. (B) Correlation curve (Spearman regression test) between the percentage of 8D4 anti–IL-4 mAb positive cells among CD3⁺ T cells and the percentage of 7-AAD⁺ cells among CD3⁺ T cells in PBMCs from controls (●) or HIV-infected patients (○) after 24-hour culture in medium. (C) Susceptibility to apoptosis (evaluated by 7-AAD staining) of MP4⁺CD3⁺ or 8D4⁺CD3⁺T cells from HIV-infected patients after 16-hour PMA+ionomycin stimulation (Mann-Whitney test).