Increased and prolonged ear swelling in DTH reactions elicited in PlGF-2 transgenic mice.

DTH reactions were induced in the ear skin of PlGF-2-transgenic and wild-type mice using oxazolone. (A) Ear swelling is expressed as the increase (Δ) over the original ear thickness in micrometers. PlGF-2 transgenic mice (●) showed a significantly increased ear swelling (P < .01) 24 hours after challenge as compared with wild-type mice (▪). Moreover, the ear swelling in PlGF-2 transgenic mice persisted longer than in wild-type mice. Unchallenged mice: ○, PlGF-2 transgenic mice; ■, wild-type mice. Data are expressed as means ± SEMs. **P < .01. Macroscopically visible increase of ear swelling and erythema in PlGF-2 transgenic mice (C) as compared with wild-type mice (B) at 24 hours after oxazolone challenge.