Increased vascular remodeling and vascular proliferation in the inflamed skin of PlGF-2 transgenic mice.

(A) Computer-assisted morphometric analysis of CD31-stained tissue sections revealed a moderately increased average vessel size at 24 hours after oxazolone challenge in the ear skin of wild-type mice (■). In contrast, cutaneous vessels in the inflamed skin of PlGF-2 transgenic mice (▪) were significantly larger than in wild-type mice after 24 hours. (B) Double immunofluorescence staining of inflamed skin of PlGF-2 transgenic mice at 24 hours after challenge for the vascular marker CD31 (red) and the proliferation marker BrdU (green) reveals proliferation of vascular endothelial cells (*). Dotted line indicates border between epidermis and dermis; c, cartilage. Original magnification × 30.