Fancc expression and mitomycin C sensitivity of BM cells from long-term reconstituted mice.

(A) Fancc Western. Whole cell lysates were extracted from the BM of long-term reconstituted mice transplanted withFancc^−/− cells transduced with either MSCV-Fancc (lane 2) or MSCV-EGFP (lane 3).Fancc^−/− murine embryonic fibroblasts transduced with MSCV-Fancc were used as a positive (+) control (lane 1). One of 4 representative blots is shown. (B) MMC progenitor assay. Six months following transplantation, BM cells were harvested from mice transplanted with either Fancc^−/− cells transduced with MSCV-Fancc (▪),Fancc^−/− cells transduced with a reporter gene only (●), or wild-type cells transduced with a reporter gene only (▴). BM cells were cultured in methylcellulose progenitor assays at 5 × 10⁴ cells/mL with increasing concentrations of MMC. Each condition was plated in triplicate and scored on day 7 of culture. The percent maximal colony formation was determined by dividing the number of colonies scored at each MMC concentration by untreated control cultures. Error bars represent standard error of the means (SEM), Student t test *P ≤ .001.