Donor chimerism of individual mice transplanted with transduced test cells.

Peripheral blood donor chimerism was determined by fluorescence cytometry 6 months following transplantation. Individual data points represent donor chimerism of single mice transplanted with eitherFancc^−/− cells transduced with a reporter gene only (−/− control), Fancc^−/− cells transduced with a retrovirus encoding rFancc (−/− rFancc), or WT cells transduced with a reporter only (+/+ control). All data from these 3 transduction groups in experiments 1-3 are shown. Mean donor chimerism of mice transplanted with −/− control cells was significantly lower than +/+ control (*P < .002). Transduction of Fancc^−/− cells with rFancc (−/− rFancc) resulted in a significant increase in repopulating ability compared to −/− control test cells (**P ≤ .04). The lower bracket outlines expected chimerism of mice transplanted with −/− control cells, and the upper bracket outlines mice (mice 1-5, 7, and 8) with a marked increase in donor chimerism. Mouse 6 in the −/− rFancc transduction group exhibited low chimerism at this timepoint but had a marked increase in chimerism 16 months after transplant. A Fisher exact test confirmed that the incidence of aberrant chimerism in the −/− control (7 of 22) transduction group was significantly greater than in the −/− rFancc (1 of 22) transduction group (P < .05).