Expression profiles for the

IGnT gene from various human tissues. Poly A⁺ RNA samples from the human brain (cerebellum), heart, small intestine, kidney, and prostate were primed using oligo-dT primer to synthesize the first-strand cDNA. Then, PCR using gene-specific forward primer and common reverse primer was performed, as described in “Patients, materials, and methods.” The RT-PCR products were analyzed using 1.5% agarose gel electrophoresis. The expected sizes of the products from the IGnTA, IGnTB, andIGnTC transcripts were 1486, 1391, and 1326 bp, respectively. From RT-PCR for the IGnTA, an additional product approximately 500 bp in size, consisting of a shorter exon 1A region conjoining with exon 2-3 regions, was observed. This smaller product is believed to result from alternative splicing and does not have a correct reading frame relative to the exon 2-3 coding sequence. The faint bands (indicated by an asterisk) below those of theIGnTA 1486-bp fragment were the hybrid complex of theIGnTA 1486-bp and the alternatively spliced 500-bp products.