![Fig. 4. The GlcNAcT activities of the enzymes encoded from the IGnTA, IGnTB, andIGnTC cDNAs. / The protein products encoded from the IGnTA, IGnTB, and IGnTC cDNAs were expressed in mammalian cells (COS-7). The amounts of GlcNAc transferred to the acceptor substrate (LS-tetrasacchride c [NeuNAca2-6Galb1-4GlcNAcb1-3Galb1-4Glc]) from the donor substrate (UDP-GlcNAc) by the medium concentrates harvested from the cells transfected with respective expression vectors were measured as has been described previously.23 The results of the average and standard deviation (indicated by error bars) of 4 tests are diagrammatically represented. Endogenous transfer of GlcNAc in the absence of acceptor substrate was corrected for each test. The amounts of the transferred GlcNAc in the vector control, pSecTaq2A, indicate the background levels of the assay, which are believed to result from the addition of the acceptor substrate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/6/10.1182_blood-2002-09-2693/4/h80634017004.jpeg?Expires=1735858359&Signature=gCEKJymFAmc9XbGxkEB0kGvUrJ15LcLASyvPBJLziWlZW9hBt9q2qdmQsN1IifDsOMEKNOoAfUd3wB~hQA4rU1tl0eTS1WEEaVIXVWYZdjTjqvHXIdBu4sYqO2zS0cSIf~x8LN-F8e8J~ff8AqzZT0JY9TE1fP3dvmR7kckjB2DO4010rS8oTQQr~wlgi-m4WkAOFaifJdKBAKJULwk16hDUfUhUghXf2cpzqukcvNPEFeCu4QZBIeXY08IAy8~pvnf8cbWkERIT0Yv9CBC2awulO08DSnTkDYOFn0QSZmVlA2tdmdo~kNrtz~2UKBsv8eCIAplIVqKQsIFvWrmCUw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The GlcNAcT activities of the enzymes encoded from the IGnTA, IGnTB, andIGnTC cDNAs.
The protein products encoded from the IGnTA, IGnTB, and IGnTC cDNAs were expressed in mammalian cells (COS-7). The amounts of GlcNAc transferred to the acceptor substrate (LS-tetrasacchride c [NeuNAca2-6Galb1-4GlcNAcb1-3Galb1-4Glc]) from the donor substrate (UDP-GlcNAc) by the medium concentrates harvested from the cells transfected with respective expression vectors were measured as has been described previously.23 The results of the average and standard deviation (indicated by error bars) of 4 tests are diagrammatically represented. Endogenous transfer of GlcNAc in the absence of acceptor substrate was corrected for each test. The amounts of the transferred GlcNAc in the vector control, pSecTaq2A, indicate the background levels of the assay, which are believed to result from the addition of the acceptor substrate.
