JunB gene expression and methylation-specific PCR.

(A) JunB gene expression levels of 3 representative CML cases as measured by real-time quantitative RT-PCR. In each CML patient, JunB expression level was higher (lower ΔC_T) at chronic phase and was lower (higher ΔC_T) at blastic crisis. (B) Methylation-specific PCR of JunB. Sss I is Sss I–treated, bisulfite-modified DNA of a healthy individual. This DNA sample was used as a positive control for methylation-specific primers. Normal is bisulfite-modifed DNA of the same healthy individual as in lane Sss I Tx. Genomic DNA is unmodified gDNA of a CML patient. M and U indicate methylation-specific PCR using JunB-M and JunB-U primer sets, respectively. CP and BC indicate chronic phase and blastic crisis of CML, respectively. Marker represents the 100-bp ladder DNA marker. Patients 1, 2, and 3 correspond to the same patients in panel A.