Composite DIC and epifluorescence images of kidney sections from engrafted animals.

The kidneys from mice with high levels of multilineage engraftment were examined microscopically. (A) A low-magnification image of a vibratome section depicts two glomeruli with associated EGFP⁺ cells. (B) A higher magnification image of a vibratome section depicts EGFP⁺ cells within the glomeruli. (C) A representative image is obtained from serial thin sections of an engrafted kidney. (D) A vibratome section of a kidney from a recipient of 100 Lin⁻, Sca-1⁺, c-kit⁺, and CD34⁻ noncultured BM cells also shows similar EGFP⁺ cells. Bars equal 25 μm.