Effect of CGP76030 on growth and survival of Bcr-Abl⁺ and AML cell lines.

CGP76030 inhibits growth and survival of Bcr-Abl⁺ but not of AML cell lines. The indicated cell lines were seeded in triplicate into 6-well plates and treated with the indicated concentrations of imatinib mesylate or CGP76030. DMSO was used as a vehicle control. The ratio of annexin-V⁺ to annexin-V⁻ cells (A) and cell counts (B) were determined 3 days later as described in “Materials and methods.”