CVX, but not collagen, treatment of RBL-2H3 cells stimulates Rap1 activation in an FcRγ-dependent manner.

RBL-2H3 cells stably transfected with wild-type GPVI (GPVI.ori), a mutant GPVI that fails to associate with FcRγ (GPVI.R272L), or untransfected RBL-2H3 cells were stimulated with 10 nM CVX. Aliquots were removed at the indicated time points. Active Rap1 was precipitated and detected as described in Figure 1. Levels of active Rap1 were determined by densitometry and normalized to total Rap1 present in each sample. These results were confirmed in 3 separate experiments.