Fig. 7.
Epinephrine augments CVX-mediated Rap1 activation in Gαi2-deficient murine platelets and 2-MeSAMP–treated human platelets.
(A) Washed Gαi2-deficient murine platelets were stimulated with either CVX or epinephrine (2 μM) or CVX and epinephrine for 1 minute. (B) Washed human platelets were pretreated with either PBS or 2-MeSAMP (2Me) for 15 minutes prior to treatment for 1 minute each with control buffers, 2 μM epinephrine (epi), 250 ng/mL CVX, or CVX and epinephrine. GTP-bound Rap1 was precipitated and detected in panels A and B as described in Figure 1, except with a polyclonal Rap1 antibody used in panel A. These results were confirmed in 3 separate experiments.
