EPO induces the secretion of TNF-α by HCD57 cells.

TNF-α enzyme immunoassay (EIA) of media harvested from HCD57 cells treated with no growth factors (lane 1), 1 U/mL EPO (lanes 2-5), or 10 ng/mL SCF (lanes 6, 7) for the number of hours indicated. TNF-α levels are measured in pg/mL compared with a standard curve using known quantities of TNF-α.