Enrichment of CD4⁺ TNK cells in TLI-treated mice.

(A) FACS contour plots of gated CD4⁺ spleen cells from a day-2 post–TLI-treated mouse (left) and a control mouse (right). The numbers in the boxed areas indicate the percentages of CD4⁺cells expressing either CD44^hi/IL-2Rβ⁺ or CD44^lo/IL-2Rβ⁻. Analysis was restricted to live cells by propidium iodine exclusion. Data are from 1 of 5 representative experiments. (B) Spleen cells from 2-day post–TLI-treated mice were analyzed for CD4, CD44, IL-2Rβ, and specific Vβ expression using 4-color FACS. Error bars represent the mean ± SE of the relative number of CD44^hi/IL-Rβ⁺ (open bar) or CD44^lo/IL-2Rβ⁻ (hatched bars) CD4 cells that stain with Vβ 8.1, 8.2, 8.3, or Vβ 8.3 from 3 separate experiments. For each experiment, 3 to 5 spleens were pulled from TLI-treated mice.