Fig. 5.
![Fig. 5. Autologous tumor cells plus IL-12–secreting bystander cells induced CD4+ T cells to proliferate upon in vitro stimulation with syngeneic tumor. / After 4 immunizations splenocytes from the indicated experimental groups were collected and purified CD4+ T cells were incubated for 5 days in the absence (■) or presence (▪) of irradiated A20 cells. [3H] incorporation is given as counts per minute (cpm) minus values for medium alone. Incorporation of irradiated A20 cells alone was comparable to that in medium. Data are represented as means (+ SDs) of the T-cell cpm per well from 3 mice in each group, analyzed individually.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/2/10.1182_blood-2002-03-0991/4/h80233650005.jpeg?Expires=1735532925&Signature=jfv9hD6ijdjiNHMJ0~f7mVtDGnYMKeN3OwMpRnwfu9NtVgCE92aCDBQxXKoAeecyHt382vQbO5HF7n9eWUoPaxyzPm0-PKwqRzwpPd7afJSdm1vbmOHrs0zo9yvRDsRdUPak7CPAd-S67xpGzK2VDU-4uQuKa9DbkMWwwLp~jaKKiBAMfaqw-7~pQHmTvVvXLD5OY5vwrP-vTUUB7fp5acWHLn1nknbThK8bYPxj6Hc5Opy8zKxUaEEtvgliJfamG3A35zqGrWZadfZc5eJQghoNhaavtsix6QKSBv1AmToSTjScepyXYXfcFt0ht~S1WUgtXJ3nxiYpQWeVY17hUA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Autologous tumor cells plus IL-12–secreting bystander cells induced CD4+ T cells to proliferate upon in vitro stimulation with syngeneic tumor.
After 4 immunizations splenocytes from the indicated experimental groups were collected and purified CD4+ T cells were incubated for 5 days in the absence (■) or presence (▪) of irradiated A20 cells. [3H] incorporation is given as counts per minute (cpm) minus values for medium alone. Incorporation of irradiated A20 cells alone was comparable to that in medium. Data are represented as means (+ SDs) of the T-cell cpm per well from 3 mice in each group, analyzed individually.
