A phagocytophila p44 DNA copy number amplified from blood.

(A) Amplification of blood was performed using quantitative PCR or (B) presented as a ratio of DNA per granulocyte in β2 integrin knock-out and B6 congenic control mice before and after I scapularis tick infestation. (A) Lines represent averageA phagocytophila p44 DNA copy number per milliliter of blood ± SE (n = 4 to 8 mice per strain per time period). *Significant differences compared with time 0 (P < .05). (B) Ratio of p44 DNA per granulocyte. This ratio was calculated by dividing total A phagocytophila p44 DNA copy number per milliliter of blood by the number of granulocytes per milliliter of blood. Columns represent average ratio ± SE (n = 4 to 8 mice per strain per time period). *Significant differences compared with time 0 (P < .05).