Fig. 6.
Hypoxia-induced increases in transcription fromEPO-Lux are mediated by Smad proteins and HIF-1α.
Confluent HepG2 cells were transfected with EPO-Lux, and cotransfected with indicated Smad and HIF-1α expression vectors, or empty vector and RSV–β-gal by electroporation. Each transfection was divided into 2 plates and exposed to either 20% or 1% O2for 24 hours. Luciferase activity was determined and results normalized to RSV–β-gal activity and protein content of the extracts. Results are expressed as fold increase in luciferase activity in hypoxic conditions compared with that found in normoxic conditions for each transfection. The figure presents the mean (±SD) of 3 independent experiments (*P < .05).
