Expression of GFP ICAM-4 and ICAM-4S fusion constructs in transfected COS-7 cells.

COS-7 cells were transfected with a GFP ICAM-4 or a GFP ICAM-4S fusion construct, fixed with 4% paraformaldehyde, and visualized using a Zeiss Axiovert 135 microscope with a 63 × 1.25 oil immersion objective. ICAM-4 (green) localized to the plasma membrane and cytoplasm (A). In contrast, ICAM-4S (green) was predominantly present in bright foci within the cytoplasm (B). Blue is DAPI (4′,6-diamidine-2′-phenylindole dihydrochloride) stain of nucleus.