Representative FACS profile of sorted CB Lin^−/lowCD34⁺CD38⁻ cells.

(A) Column-enriched CD34⁺ cells were stained with anti-CD45 mAb and antilineage-specific mAb cocktail and were gated on lineage marker–negative and/or low expression region (R1). (B) R1 cells were further gated on CD34⁺CD38⁻ region (R2). (C) Re-analysis of isolated CD34⁺CD38⁻ cells from R2 (99.8% purity).