Scianna genotyping by PCR-SSP.

PCRs were devised to amplify specific PCR products of 147-bp size from the wild-type ERMAP allele representative of Sc1 (A) or from the ERMAP(Gly57Arg) allele carrying the Sc2 antigen (B), respectively. Representative results for Sc:1,-2; Sc:1,2; Sc:-1,2; and Sc:-1,-2 samples are shown. Control PCR products of 434-bp size occurred in all reactions.