Effect of L-fucose therapy on rolling in vivo of LADII neutrophils in cremasteric venules of NOD/SCID mice.

Neutrophils purified from a healthy donor or the LADII patient were fluorescently labeled and injected through the carotid artery into NOD/SCID mice prepared for intravital microscopy of the cremaster muscle. (A) The percentage of interacting LADII neutrophils obtained on days 0 and 8 of treatment was greatly reduced compared with that of neutrophils from a healthy donor. On day 16, however, a sharp increase in the number of interactions of the patient's neutrophils was detected. Error bars represent mean ± SEM. n = 4-6 venules per mouse. * P < .01 compared with healthy or day 16. (B) Altered kinetics in neutrophil rolling on day 16. Detailed analysis of the time and length of each rolling event from healthy (▪) or LADII (○) neutrophils from day 16 reveals transient interactions of LADII neutrophils compared to those from a healthy donor.