Role of PECAM-1/CD31 and HS as endothelial receptors for pRBCs of FCR3S1.2.

(A) Adhesion of pRBCs was tested on nontreated HUVECs (♦) or after pretreatment (▪) with heparinase III (0.2 IU/mL for 2 hours) in the presence of anti–PECAM-1/CD31 antibody at the indicated concentrations. All samples were compared with controls in which pRBCs alone were incubated with nontreated HUVECs. (B) DBL1α-GST (●) or GST alone (■) was incubated with HUVECs for 1 hour before pRBCs were added to HUVECs and allowed to bind for 1 hour. All data are expressed as the mean of 3 independent experiments ± SD.