Fig. 5.
![Fig. 5. Binding of endothelial HS to DBL1α, CIDR1α, or DBL-2δ. / Human endothelial [35S]HS, bovine lung [3H]heparin, and human endothelial [35S]CS were incubated on columns with immobilized DBL1α-GST (panels A-C), CIDR1α-GST (panels D-F), and DBL2δ-GST (panels G-I) for 20 minutes followed by washing with TBS and elution with a stepwise gradient of increasing salt concentration in the same buffer. Then, 1-mL fractions were collected and analyzed for radioactivity. Control experiments carried out with GST NHS-sepharose did not show any binding (not shown).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/6/10.1182_blood-2002-07-2016/4/h80634014005.jpeg?Expires=1735525853&Signature=azKl15ePwbs2STTQlWAR2o~kkUOJJXgDBnsh44FDMoyxrOASnaUnrwWkodi0NFTb4EbbSpUucuN6HglEQYcblTTMWbU8aAr-~SIIBUm31JMk4TPLdwu3uAoEQ0dUfWT1Btjc-XKhcWlZyXClKr37aECBBYGqlddR5s3BHaJZw5Ngo7yaRMPFe1onbbwVEhC4RKoZ71OekmrnvGtmbmGokA8~PzrWhptIoa6aWOunTY92GMGYd6gZ7kZhrv9HJcdtPPM-r3MInY54RXd0kFd2op7BXCPatSchB9AWYOnxy5VLFAZwCrTBjxj9ZTEDsMzhJSgsBzr~WGakkkpnX4le0Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Binding of endothelial HS to DBL1α, CIDR1α, or DBL-2δ.
Human endothelial [35S]HS, bovine lung [3H]heparin, and human endothelial [35S]CS were incubated on columns with immobilized DBL1α-GST (panels A-C), CIDR1α-GST (panels D-F), and DBL2δ-GST (panels G-I) for 20 minutes followed by washing with TBS and elution with a stepwise gradient of increasing salt concentration in the same buffer. Then, 1-mL fractions were collected and analyzed for radioactivity. Control experiments carried out with GST NHS-sepharose did not show any binding (not shown).
