Genomic structure of mouse

Evi3 and AKXD27 tumor analysis. (A) Genomic organization of Evi3. Exons are indicated as boxes; open boxes are 5′ or 3′ untranslated regions, whereas filled boxes are coding. Introns are indicated by lines between the exons, and intron sizes are given below. The exons are numbered below the boxes, and relative sizes of exons are drawn to scale. The sizes of the introns are not drawn to scale. The position of 8 retroviral integrations are depicted as filled arrowheads, with the arrow indicating the orientation relative to the Evi3 gene. (B) Southern blot analysis of SacI-digested genomic DNA from 7 AKXD27 tumors, hybridized with an Evi3-specific probe. A 15-kb germ line band is seen in all lanes, and a 6.7-kb band resulting from clonal viral integration is seen from tumors 22, 27, and 29. (C) Strategy for IPCR cloning of genomic sequence flanking the retroviral insertion sites, with the use of SacI-digested DNA and Evi3genomic nested primers (arrows).