Evi3 expression in AKXD27 tumors and normal B cells.

(A) RT-PCR analysis of normal Evi3 expression in different B-cell subsets (upper panel). Exon 3 and exon 4 primers were used. FL indicates fetal liver; BM, bone marrow. Pro-B indicates IgM⁻IgD⁻CD43⁺B220⁺cells; Pre-B, IgM⁻IgD⁻CD43⁻B220⁺cells; Imat B (immature B), IgM⁺IgD⁻ cells; Mat B (mature B), IgM⁺IgD⁺ cells. Lower panel shows the result from using Hprt control primers. (B) RT-PCR analysis of Evi3 expression in the 7 AKXD27 tumors. Upper panel shows the results from RT-PCR with 2 Evi3 primers as in panel A. The lower panel shows the results of RT-PCR withHprt control primers. (C) RNase-protection analysis of RNA from the 7 AKXD27 tumors with an Evi3-specific probe (upper panel) or an internal control probe (Cyclophilin, lower panel). For clarity, only the protected bands are shown.