Fig. 3.
Fig. 3. Down-regulation of TCR after stimulation with HLA A24–positive LCLs demonstrated by decreased levels of tetramer staining. / Before staining with each MHC/peptide tetramer (eg, Tet-11 means tetramer incorporating peptide no. 11), aliquots of EBV-specific CD8+ T cells were incubated with HLA A24–positive LCLs (top row), HLA A24–negative LCLs (middle row), or HLA A24–positive CD40-activated B cells (bottom row). After a 16-hour incubation, the cells were stained with PE-conjugated HLA A*2402 tetramers and TRI-COLOR–labeled anti-CD8 mAb, and analyzed by FACSCalibur. Numbers in the top right quadrants represent the percentages of tetramer-positive cells in the total CD8highcells.

Down-regulation of TCR after stimulation with HLA A24–positive LCLs demonstrated by decreased levels of tetramer staining.

Before staining with each MHC/peptide tetramer (eg, Tet-11 means tetramer incorporating peptide no. 11), aliquots of EBV-specific CD8+ T cells were incubated with HLA A24–positive LCLs (top row), HLA A24–negative LCLs (middle row), or HLA A24–positive CD40-activated B cells (bottom row). After a 16-hour incubation, the cells were stained with PE-conjugated HLA A*2402 tetramers and TRI-COLOR–labeled anti-CD8 mAb, and analyzed by FACSCalibur. Numbers in the top right quadrants represent the percentages of tetramer-positive cells in the total CD8highcells.

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