Fig. 6.
Results of ELISPOT assays using CD8+ T-cell clones.
IYV-CTL is a clone specific for peptide no. 11, IYVLVMLVL; RYS-CTL, a clone specific for peptide RYSIFFDYM. LCL1 and 2 were established from PBMCs of HLA A24–positive donors, and LCL3 and 4 from HLA A24–negative donors. Abbreviations used for describing the stimulators are as follows: IYV, T2-A24 cells pulsed with peptide IYVLVMLVL; RYS, T2-A24 cells pulsed with peptide RYSIFFDYM; vL2, cells infected with vLMP2; vE3A, cells infected with vEBNA3A. CD8+ T cells (200 [▪]; 100 [░]; 50 []; or 25 [■]) were cultured with the indicated APCs in each well. Each bar represents the average number of spots in duplicate wells.