Fig. 2.
![Fig. 2. Beta-galactosidase (β-galactosidase)–specific activity of BVL-1–like, ELM 5 promoter shows sustained expression patterns in transfected ELM clones. / The VL30 promoters ELM 5, MEL/ELM, negative control NVL3 promoter within VLIBAG, and the positive retroviral control vector, RVBAG, were transfected into ELM-I-1 cells and monitored for β-galactosidase reporter gene activity through ONPG hydrolysis assays over a 16-week time course. β-Galactosidase reporter gene activity was monitored in cell lysates from all 4 constructs in epo-induced (10 U/mL epo) growth conditions. The transfected BVL-1–like ELM 5 vector showed sustained β-galactosidase activity and modest epo induction (*P < .05, n = 6 [error bars]) on weeks 4, 8, 10, 12, 14, and 16.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/5/10.1182_blood-2002-07-2105/3/h80533928002.jpeg?Expires=1735543934&Signature=TtRHHjvDiMM2sr5pVssVjE443VitdGyXfJ~jwYnqEOCt07E038Nf5SaZfjyF~hviCjnlmZy5SzcPKg6u3vKkGAFVC7Z8ItYb129z8mXkgXH9m17A26Pxg3X~Lnn3JyCBK146oXjyjj0~nVSKcTg7hUAM3clkHBNPZmqaQs1XQtEo1Mc5yCF1oF~Q2hdnEzrLZE4YSrbp7MQhJUwQe0aiCmc5EiotYMvM9H2R06xSSuzzku8T7MD4QGAknhINOSygbbtamurjSckV550YpgbRq04XgvrJdPGC-31lCoJ~ihHCK5Bc7i84WxHYdgGFTjoKF1ppxoTOF9zP8iVlysmEfA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Beta-galactosidase (β-galactosidase)–specific activity of BVL-1–like, ELM 5 promoter shows sustained expression patterns in transfected ELM clones.
The VL30 promoters ELM 5, MEL/ELM, negative control NVL3 promoter within VLIBAG, and the positive retroviral control vector, RVBAG, were transfected into ELM-I-1 cells and monitored for β-galactosidase reporter gene activity through ONPG hydrolysis assays over a 16-week time course. β-Galactosidase reporter gene activity was monitored in cell lysates from all 4 constructs in epo-induced (10 U/mL epo) growth conditions. The transfected BVL-1–like ELM 5 vector showed sustained β-galactosidase activity and modest epo induction (*P < .05, n = 6 [error bars]) on weeks 4, 8, 10, 12, 14, and 16.
