Neutrophil populations in IkL/L mice.
(A) Phenotypic analysis of WT and IkL/L FL and BM cells using the Gr-1 and Mac-1 markers. Numbers shown correspond to the percentage of cells in each gate. The experiment shown is representative of 3 experiments. (B) Absolute number of total (left) and Mac-1+ (right) FL cells corresponding to fetuses analyzed in Figure 2A. Note that because of the large variability in FL cellularity between fetuses from different litters of the same developmental stage, representative results from individual WT and IkL/L littermates are shown here. (C) Absolute numbers of total BM cells (left) and Mac-1+ cells (right) per femur in 6-week-old mice (n = 11 for WT; n = 13 for IkL/L). (D) Absolute numbers of neutrophil lineage cells in the blood of mice treated or not with G-CSF. (** indicates P < .01, n = 11; *, P < .05, n = 4).
