Neutrophil function is not altered in Ik^L/Lmice.

(A) Appearance of Mac-1⁺Gr-1⁺ cells in the peritoneal cavity 20 hours after intraperitoneal LPS injection. Numbers represent the percentage of Mac-1⁺Gr-1⁺ cells. (B) Numbers of BM cells that had migrated from the upper chamber of a transwell apparatus to the lower chamber containing IL-8 after a 90-minute culture. Bars indicate the means ± SDs of triplicate cultures for each genotype of one representative experiment. (▪) indicates WT; (■) indicates Ik^L/L. (C) Phagocytic activity of WT and Ik^L/L blood cells as measured by the uptake of opsonized and FITC-conjugated E coli at 37°C for 1 hour. The proportion of internalized bacteria was subsequently measured by flow cytometry. (D) NADPH oxidase-mediated O$2−$ burst was evaluated in BM cells after incubation with immune complexes bound to H₂DCF for 30 and 45 minutes at 37°C. Following internalization of the H₂DCF, O$2−$ levels were assayed as a function of fluorescence emission by the reduced DCF by flow cytometry. Samples were run in triplicate and the results of a representative experiment are presented as means ± SDs. ▪ indicates WT; ■, Ik^L/L. One representative experiment is shown for each panel of Figure7.