Effect of NAC and rotenone on TNF-α/zVAD-fmk–induced PMN cell death.

PMNs were cultured for 6 hours with a combination of 20 ng/mL TNF-α and 150 μM zVAD-fmk or with TNF-α/zVAD-fmk in combination with 5 mM NAC or 100 μM rotenone. Afterwards, PMNs were stained with annexin-V/PI and analyzed by FACScan. Values represent the percentage of cells (mean ± SEM) for each respective quadrant. Data obtained in 5 independent experiments.