Fig. 4.
Fig. 4. Bridge formation between CD8+ T cells and merozoites by 5.2-OKT3 biscFv. / Schizont-stage parasites were enriched by treatment with E64 as described in “Materials and methods.” To release merozoites from its parasitophorous vacuole, E64 was removed completely from culture. The parasites were incubated for 3 to 5 hours in the presence of 50 μg/mL 5.2-OKT3 biscFv. When merozoites were detected in the culture, CD8+ T cells were added and incubated for one hour. (A) Bridge formation between CD8+ T cells and merozoites was detected by indirect immunofluorescence staining with FITC-conjugated anti-FLAG M2 mAb. (B) The same image is shown with DAPI staining and photographed through a fluorescence microscope. The nuclei of CD8+ T cells and merozoites were stained in blue. Original magnification, × 1000.

Bridge formation between CD8+ T cells and merozoites by 5.2-OKT3 biscFv.

Schizont-stage parasites were enriched by treatment with E64 as described in “Materials and methods.” To release merozoites from its parasitophorous vacuole, E64 was removed completely from culture. The parasites were incubated for 3 to 5 hours in the presence of 50 μg/mL 5.2-OKT3 biscFv. When merozoites were detected in the culture, CD8+ T cells were added and incubated for one hour. (A) Bridge formation between CD8+ T cells and merozoites was detected by indirect immunofluorescence staining with FITC-conjugated anti-FLAG M2 mAb. (B) The same image is shown with DAPI staining and photographed through a fluorescence microscope. The nuclei of CD8+ T cells and merozoites were stained in blue. Original magnification, × 1000.

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