Fig. 5.
Production of IL-2, TNF-α, and IFN-γ from mononuclear cells stimulated with scFvs.
(A) ScFvs (100 μg/mL) were preincubated with rPfMSP-119–coated 96-well plates. After washing, PBMCs (2 × 105/well) were added and cultured for 24 hours. Culture supernatants were harvested and assayed for production of IL-2, TNF-α, and IFN-γ by a sandwich ELISA. (B) Highly synchronous blood-stage cultures of P falciparum at an initial parasitemia of 1% were cocultured with PBMCs (2 × 105/well) and scFvs (50 μg/mL) for 24 hours in a 96-well plate. Culture supernatants were assayed as described in panel A. Results are presented as the means ± SDs from 3 separate experiments. *P < .01 compared with 5.2 scFv and OKT3 scFv. ND indicates not detectable.
