Fig. 1.
Concentration of AcSDKP in cultured EA.hy926 cells.
The intracellular concentration of AcSDKP (plotted line) was measured daily in EA.hy926 cells grown to confluence in 75-cm2 flasks. Following trypsin detachment, the cells were counted (full bars), washed twice with culture medium, sonicated, and AcSDKP extracted with methanol according to the procedure described in “Materials and methods.” The extracellular level of AcSDKP was evaluated in the supernatants collected from EA.hy926 cell layers grown in complete medium for 2 and 7 days and then cultured for 24 hours in the serum-free medium. The results are the mean ± SEM of 4 experiments.
