Effect of a biotinylated polymeric, N-acetyl-galactosamine–containing clearing agent on circulating BC8-SA and 1F5-SA conjugates.

Two groups of 10 BALB/c athymic mice were injected intraperitoneally with 1.4 nmol ¹³¹I-1F5-SA conjugate (closed symbols) or¹³¹I-BC8-SA conjugate (open symbols) at time 0. Five mice from each group were injected intraperitoneally 24 hours later with 5.8 nmol clearing agent (+CA; triangles), whereas the other 5 mice in each group did not receive clearing agent (−CA; squares). Serial blood samples were obtained from the retro-orbital venous plexus 25, 26, 48, and 72 hours after the injection of either mAb-SA conjugate and were analyzed by gamma counting.