Effect of donor pretreatment on spleen phenotype.

Naive B6 mice were treated with control diluent (■), G-CSF (10 μg/animal per day for 10 days, ▨), or ProGP-1 (20 μg/animal per day for 10 days, ▪). Spleens were harvested on day 11, chopped, digested, and phenotyped. DCs were either CD11c^dim/B220^hi or CD11c^hi. (A) Proportion of lineage cells per spleen. (B) Absolute numbers of lineage cells per spleen. *P < .05 compared with controls.