Fig. 2.
Effect of cytokine pretreatment on splenic DC phenotype.
Naive B6 mice were treated with control diluent, G-CSF, or ProGP-1 as described in “Materials and methods.” DCs were enriched as described, presorted to remove autofluorescent macrophages (A), and stained with CD11c and B220 (B). The CD11chi DCs (R1) from control spleen (C), G-CSF spleen (D), and ProGP-1 spleen (E) were further analyzed for CD4 and CD8 expression.
