Effect of cytokine pretreatment on splenic T-cell phenotype.

Naive B6 mice were treated with control diluent, G-CSF, or ProGP-1 as described in the legend to Figure 3. Splenocytes were harvested and digested, and CD3⁺ T cells were examined for their expression of CD4, L-selectin, CD44, and CD25 by 3-color flow cytometry.