ProGP-1–expanded donor DC populations fail to confer protection from GVHD.

Survival curves by Kaplan-Meier analysis, pooled from 2 similar experiments. Donor B6 mice were treated with ProGP-1 or control diluent. Splenocytes were harvested on day 11 and control splenocytes were transplanted into lethally irradiated B6D2F1 recipient mice at doses of 10⁷/animal (▪, allogeneic, n = 10). ProGP-1–expanded CD11c^hi DCs (▵, n = 10) or CD11c^dim/B220^hi DCs (⋄, n = 5) were added to control allogeneic splenocytes in numbers equivalent to those in unseparated ProGP-1 spleen (10⁶CD11c^hi and 2.5 × 10⁵CD11c^dim/B220^hi). Syngeneic spleen (○, syngeneic, n = 3) was transplanted as a non-GVHD control.