![Fig. 1. Immunofluorescence of stromal cells from hematopoietic fetal liver. / (A) A 14-gw human fetal liver; (B) 14.5-dpc murine fetal liver; (C) AFT024 cell line from 14-dpc liver; and (D) 2018 cell line from 14-dpc liver. Fetal liver cells were obtained and cultured for 10 days in long-term culture medium; cells were then fixed and double-immunostained with Cy3-conjugated mouse anti-ASMA (red) and mouse anti–CK-8 (green; fluorescent secondary antibody was fluorescein isothiocyanate [FITC]–conjugated goat antimouse immunoglobulin). Panels show an overlay of the fluorescence signal from the 2 channels (red and green) to visualize a coexpression of the epithelial and mesenchymal marker. Expression of ASMA in cell lines is so intense that the signal appears yellow instead of red. Scale bar = 10 μm.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/8/10.1182_blood-2002-05-1341/4/h80834152001.jpeg?Expires=1735530388&Signature=EMfu~QeWcuXFSmZrKL7mT6nwefnSHN7IDV9U~xLEurUWrmE1I8u~X4f0HTz7MXd9rMwbP0Mwri~AF1qckio~hZvsHkvdwENgxpASKKNk-mte9YmoLTl0Aso5ZZyDQ4Hrqt2j90TVxfoGWyVu1a-Hseddtt3xxHirlpinOkX2d3NqzzwVsIfG1NvI86Z5-7uqFsSAXXvPQb9tjv4SlDyQuhqxMxXMsUuoFi1eEWyWE67d6Kw-A13wxrCrMtPVKLlftHzB~oR4Grf2H53aB7acd6HPT5IYXyFfZo5qNd~f70yoyFDgKD65sShCTwDXy7uXvLu4SLz2cC9PGBr21TzSBw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunofluorescence of stromal cells from hematopoietic fetal liver.
(A) A 14-gw human fetal liver; (B) 14.5-dpc murine fetal liver; (C) AFT024 cell line from 14-dpc liver; and (D) 2018 cell line from 14-dpc liver. Fetal liver cells were obtained and cultured for 10 days in long-term culture medium; cells were then fixed and double-immunostained with Cy3-conjugated mouse anti-ASMA (red) and mouse anti–CK-8 (green; fluorescent secondary antibody was fluorescein isothiocyanate [FITC]–conjugated goat antimouse immunoglobulin). Panels show an overlay of the fluorescence signal from the 2 channels (red and green) to visualize a coexpression of the epithelial and mesenchymal marker. Expression of ASMA in cell lines is so intense that the signal appears yellow instead of red. Scale bar = 10 μm.
