Flow cytometric analysis of the in vitro differentiation of ES cells into VEGFR2⁺ mesodermal cells.

CCE ES cells were cultured on type IV collagen-coated dishes in the absence of leukemia inhibitory factor. (A, left panel) VEGFR2⁺ E-cadherin–negative cells were generated after 4 days for ES cell differentiation. In this experiment, the proportion of this population reached 31.2%. (A, right panel) The VEGFR2⁺ E-cadherin-negative cells were sorted and reanalyzed. (B) The expression of PECAM-1 and TIE2 in the purified VEGFR2⁺ cells were analyzed. The numbers indicate the percentage of cells that appeared in each quadrant.