Flow cytometric analysis of the in vitro differentiation of sorted VEGFR2⁺ cells into EC lineage under a chemically defined condition.

Sorted VEGFR2⁺ cells were cultured on type IV collagen–coated dishes under a chemically defined condition without additional factors (A), or with 50 ng/mL VEGF (B). The expression of VE-cadherin, PECAM-1, TIE2, and CD34 was analyzed after 3 days of culture. The numbers indicate the percentage of cells that appeared in each quadrant.