Flow cytometric analysis of the in vitro differentiation of sorted VEGFR2⁺ cells into EC lineage under a chemically defined condition.

Sorted VEGFR2⁺ cells were cultured on type IV collagen–coated dishes under a chemically defined condition in the presence of serially diluted VEGF. The expression of VE-cadherin and CD34 was analyzed after 4 days of culture. The numbers indicate the percentage of cells that appeared in each quadrant.