Flow cytometric analysis of the in vitro differentiation of sorted VEGFR2⁺ cells into EC lineage under a chemically defined condition.

Sorted VEGFR2⁺ cells were cultured on type IV collagen–coated dishes under a chemically defined condition in the presence of 30 ng/mL VEGF. The expression of VEGFR1 and VEGFR2 was analyzed before cell sorting (day 0) and after 2 days of culture (day 2). The numbers indicate the percentage of cells that appeared in each quadrant. The change of VEGFR1 expression in VEGFR2⁺population was shown in a histogram (lower panel).