Order of intron splicing in the FGA gene.

(A) RT-PCR products obtained for reactions C-L were separated on a 1% agarose gel. For each reaction, a PCR amplification was performed without prior reverse transcription (RT−) to exclude DNA contamination. The splice intermediates corresponding to each band and the deduced order of intron splicing are detailed in Table 2. M denotes the 1-kb ladder DNA size marker. (B) RT-PCR was performed for reactions C and D with radioactively end-labeled sense primers on nuclear RNA intermediates isolated after actinomycin treatment (0, 5, 10, 20, 40, and 60 minutes). The products were separated on 6% denaturing polyacrylamide gels. PCR products amplified from genomic DNA were used as size markers.