Electroporation of Kasumi-1 cells with a fluorescently labeled siRNA.

(A) FACS analysis of electroporation efficiency. Kasumi-1 cells were electroporated as described in “Materials and methods.” Cells were analyzed by FACS 16 hours after electroporation. White peak, cells electroporated without siRNA; black peak, cells electroporated with 200 nM 5′Cy3-labeled siGL2. (B) Intracellular distribution of electroporated siRNAs. 16 hours after electroporation in the presence of 200 nM 5′Cy3-labeled siGL2, intracellular fluorescence was analyzed by fluorescence microscopy. Original magnification × 610. Upper panel, Cy3-labeled siRNA; lower panel, DAPI (diamidino-phenyl-indol) staining of cell nuclei.