American Society of Hematology

Fig. 1.

$Fig. 1. SDS-PAGE analysis of purified sGMRα and sβc. / Soluble GMRα and sβc were produced by Sf21 cells infected with recombinant baculovirus encoding appropriate cDNA and affinity purified from the supernatant as described in “Materials and methods.” Soluble GMRα (1 μg) and sβc (0.5 μg) were fractionated by 10% SDS-PAGE under reducing (A) and nonreducing (B) conditions and silver stained. The positions of molecular weight markers are shown in kilodaltons.$

SDS-PAGE analysis of purified sGMRα and sβc.

Soluble GMRα and sβc were produced by Sf21 cells infected with recombinant baculovirus encoding appropriate cDNA and affinity purified from the supernatant as described in “Materials and methods.” Soluble GMRα (1 μg) and sβc (0.5 μg) were fractionated by 10% SDS-PAGE under reducing (A) and nonreducing (B) conditions and silver stained. The positions of molecular weight markers are shown in kilodaltons.

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